Distribution of CCR5 Δ32 HIV-1 resistance allele frequency at Zanjan province in Iran

Introduction: The CC chemokine receptor 5 (CCR5) is known as the main co-receptor in human immunodeficiency virus (HIV) infection. Accumulating evidence verified 32bp deletion both alleles of CCR5 provides natural resistance to HIV Accordingly, recent therapeutic approaches are based on gene and cell therapies by inducing this resistance. Allogeneic transplantation stem cells with CCR5Δ32 homozygous infected people has been considered an efficient strategy. Objectives: aim study was determine Δ32 mutation frequency healthy HIV-infected individuals Zanjan province, Iran also detecting appropriate candidate for future approaches. Patients Methods: In study, blood samples were collected from 102 patients 204 controls ethylene diamine tetra-acetic acid (EDTA) pre-coated tubes. DNA extraction performed analyzed GAP-PCR groups followed 2% gel agarose electrophoresis. genotype statistically determined. Results: Our finding demonstrated that 3 displayed mutation, while showed one Δ32/Δ32 homozygote mutation. We observed 0.82% province (χ 2 =47.58, P <0.001). Conclusion: Reduction well other polymorphisms related resistance, can lead development infection epidemiology. Migration, selection genetic admixture elucidate various distribution allele different populations, like north Europeans reduce gradually south. result facilitate presentation suitable donors